The Wolfeboro Railroad or Wolfeborough Railroad (later the Wolfeboro Branch of the Boston and Maine Railroad) is a former short line that provided service to the summer resort town of Wolfeboro, New Hampshire (formerly spelled “Wolfeborough”) on Lake Winnipesaukee.

In 1979, the Wolfeboro Steam Railroad Corporation bought the Wolfeboro line, running tourist trains from 1980 until 1985. In 1985, the line was purchased by the State of New Hampshire, which uses the right-of-way as the Wolfeboro Recreational Rail Trail, now known as the Cotton Valley Rail Trail. The rails are intact, and they are actively used by rail clubs. The rail corridor is maintained by members of the Cotton Valley Rail Trail Club. In 1987, Wolfeboro Station was struck by lightning and caught fire, and it was repaired by the town.